PROTEIN BIOSYNTHESIS BY A CELL-FREE BACTERIAL SYSTEM
نویسندگان
چکیده
منابع مشابه
Chlorophyll biosynthesis in a cell-free system from higher plants.
A crude homogenate obtained from greening cucumber (Cucumis sativus, L.) cotyledons in tris-sucrose, pH 7.7, containing coenzyme A, glutathione, potassium phosphate, pH 7.7, methyl alcohol, magnesium, nicotinamide adenine dinucleotide, and adenosine triphosphate, is able to incorporate 4-(14)C-delta-aminolevulinic acid into chlorophyll a and b in the presence of oxygen. If the homogenates are p...
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The investigation reported in this communication was concerned with the biosynthesis of ferredoxin in a cell-free system. A cell-free system, with the ability to incorporate amino acids into peptides and proteins, was developed from Cl. pasteurianum, and its requirements were established. After the incorporation of L-alanine-C(14), ferredoxin-C(14) was isolated in crystalline form with the aid ...
متن کاملProtochlorophyll biosynthesis in a cell-free system from higher plants.
A cell free system prepared from etiolated cucumber (Cucumis sativus, L) in tris-sucrose buffer is able to incorporate delta-aminolevulinic acid-4- (14)C into the two components of protochlorophyll: protochlorophyllide and protochlorophyllide ester. The activity is associated with the etioplasts. Optimal incorporation is obtained at pH 7.7. For the formation of protochlorphyllide ester, oxygen,...
متن کاملThe Biosynthesis of Gramicidin S in a Cell-free System.
1. A cell-free system prepared from Bacillus brevis cells, harvested in the late phase of growth and consisting of the 11000g supernatant, has been shown to incorporate into gramicidin S the five constituent amino acids added in labelled form. The results are consistent with complete synthesis and not merely a completion of pre-existing intermediate peptides. 2. The incorporation of (14)C-label...
متن کاملThe Biosynthesis of Chondroitin Sulfate by a Cell-free Preparation.
The biosynthesis of the acid mucopolysaccharide, hyaluronic acid, has been demonstrated in cell-free preparations derived from Group A streptococci (1, 2) and embryonic rat skin (3). In keeping with their function as glycosyl donors in many systems, the uridine nucleotides, uridine 5’-diphosphate-N-acetyln-glucosamine and UDP-n-glucuronic acid, have been shown to be the requisite intermediates ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1958
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.44.6.494